| Title | [Protection effects of acetylcholinesterase inhibitors on apoptosis] | | Author(s) | Huang J, Yi Y, Bi SL, Chen SY, Wang CY | | Institution | Beliing An Ding Hospital, Capital Medical University, Beijing 100088, China. | | Source | Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2008 Dec; 22(6):458-60. | | Abstract | OBJECTIVE: To investigate whether tacrine and donepezil can prevent apoptosis induced by Lipopolysaccharides.
METHODS: Phase-contrast microscopes was used to observe the morphological changes of Vero cells. Cell counting kit-8 was used to measure cell survival vitro. DNA fragment was analyzed by agarose gel electrophoresis to determine the apoptotic biochemical changes.
RESULTS: Vero cells treated with 400 microg/ml and 500 microg/ml Lipopolysaccharides exhibited cell apoptosis. 10 micromol/L tacrine provided protective effect to 500 microg/ml Lipopolysaccharides induced cell apoptosis measured by Phase-contrast microscopes, cell counting kit-8 and DNA fragment analyze. However, donepezil did not show any protective effect of the apoptosis induced by 500 microg/ml Lipopolysaccharides.
CONCLUSION: Lipopolysaccharides can induce apoptosis in Vero cells to built an apoptotic model in vitro. Tacrine rather than donepezil can inhibit Lipopolysaccharides induced apoptosis in Vero cells. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 19544645 |
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